Plasmid Curing and Generation of Mutations Induced with Ethidium Bromide in Streptomycetes
نویسندگان
چکیده
منابع مشابه
Ethidium Bromide Induced Histological Changes in Spleen of Albino Mice
Adult Mice (BWt. 30 gms and 40gms) were treated at a 5mg\kgBWt. and 10 mg/kg BWt. of Ethidium Bromide for 10 days in drinking water. Control animals were given equal dose of deionised water. Quantitative and qualitative changes were studied in Spleen. The following features were evaluated: the overall cellularity of the spleen was noticed. The changes were characterised by, injury to spleen tis...
متن کاملViewing of complex molecules of ethidium bromide and plasmid DNA in solution by atomic force microscopy.
Tertiary structure changes in plasmid DNA, induced by ethidium bromide intercalation, have been observed in aqueous solutions by the use of an atomic force microscope. A relaxed closed circular pBR322 molecule became a positively supercoiled complex on the drug binding. The supercoiling always resulted in an interwound (or a plectonemic) form, but never a solenoidal (or a toroidal) form. A quan...
متن کاملA novel and robust conditioning lesion induced by ethidium bromide.
Molecular and cellular mechanisms underlying the peripheral conditioning lesion remain unsolved. We show here that injection of a chemical demyelinating agent, ethidium bromide, into the sciatic nerve induces a similar set of regeneration-associated genes and promotes a 2.7-fold greater extent of sensory axon regeneration in the spinal cord than sciatic nerve crush. We found that more severe pe...
متن کاملEffects of ethidium bromide on growth and on loss of the penicillinase plasmid of Staphylococcus aureus.
Ethidium bromide (EB) was more efficient than ethyl violet or rifampin as a curing agent for the penicillinase plasmids of Staphylococcus aureus strains. The effects of EB on growth and on the loss of the penicillinase plasmid of PS 81 were studied in detail. The growth rates of PS 81 and an EB-cured derivative were identical in broth, but the cured derivative had a shorter lag in the presence ...
متن کاملEffects of Crocin on Oxidative Stress Indices in Rat Brain Demylated with Ethidium Bromide
Background and purpose: The purpose of this study was to investigate the effects of crocin (Cr) on brain oxidative stress indices in animal model demyelinated with intracerebroventricular ethidium bromide (EB). Materials and methods: In an interventional study, female Wistar rats were randomly divided into 7 groups. Crocin administration was done 1 day after EB injection for 21 days. The value...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microbiology
سال: 1986
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-132-3-819